乳果糖和富氢水对断奶仔猪采食霉变玉米后引起卵巢机能障碍的缓解作用

doi:10.11843/j.issn.0366-6964.2018.12.013

Abstract

Abstract:

The objective of the present study was to investigate the effects of contaminated corn by Fusarium graminearum on the antioxidant levels and NOS of ovary of weaned piglets and the mitigation of lactulose and hydrogen-rich water. Twenty-four weaned piglets of three-hybrid (Duroc×Landrace×Large White) of 21 days old with average body weight (7.25±1.02) kg were randomly divided into 4 groups (control group, contaminated corn group, lactulose relieving group, hydrogen-rich water relieving group), and 6 weaned piglets in each group. The piglets in control group were fed the basal diet, while piglets in contaminated corn, lactulose relieving and hydrogen-rich water relieving groups were fed the moldy feed with the deoxynivalenol (DON) and zearalenone (ZEA) contents of 0.4 mg·kg^-1 and 0.55 mg·kg^-1, respectively. In addition, the lactulose and hydrogen-rich water relieving groups were additionally intragastrically administered twice daily with lactulose (500 mg·kg^-1) and hydrogen-rich water (10 mL·kg^-1), respectively. The experiment lasted for 25 days. After 25 d, piglets were slaughtered, ovaries were collected and weighed. The ovary of piglets was observed histologically, the activities of CAT, GSH-PX, T-SOD, SDH, Caspase-3, NOS and content of NO in ovary were detected. The expressions of nNOS, iNOS and eNOS were also detected by immunohistochemistry. The results showed that:1) The relative ovary weight of the piglets in the contaminated corn group was significantly higher than that in the control group (P<0.05). Compared with the contaminated corn group, the relative ovary weight of piglets in the lactulose relieving group and the hydrogen-rich water relieving group had the downward trend. 2) There were no obvious pathological changes in the ovary of piglets in the 4 groups, but the number of growing follicles in the contaminated corn group were significantly higher than those in the control group (P<0.05). The number of growing follicles in the lactulose and hydrogen-rich water relieving groups decreased(P>0.05) compared with the contaminated corn group. 3) Compared with the control group, the activity of CAT in the contaminated corn group increased significantly, the activities of GSH-PX and SDH decreased significantly (P<0.05) and the T-SOD activity had a decreasing trend, the Caspase-3 activity, NO content, total NOS activity significantly increased (P<0.05). 4) Compared with the contaminated corn group, GSH-PX and SDH activities in the lactulose relieving group significantly increased (P<0.05). GSH-PX and SDH activities in the hydrogen-rich water relieving group had a tendency to increase; CAT activity in lactulose and hydrogen-rich water relieving groups decreased(P>0.05), T-SOD activity increased(P>0.05); Caspase-3 activity and NO content in lactulose and hydrogen-rich water relieving groups decreased significantly (P<0.05). Total NOS activity in lactulose relieving group had a tendency to decrease, while it in hydrogen-rich water relieving group decreased significantly (P<0.05). 5) The results of immunohistochemistry showed that three kinds of NOS (nNOS, iNOS, eNOS) were localized in oocytes, granulosa cells and theca cells of follicles at all stages, and showed cell-specific expression. DON and ZEA in contaminated corn may mediate the decrease of anti-oxidation levels in piglet ovaries, which leads to deeper apoptosis, while lactulose and hydrogen-rich water effectively relieve the harmful effects of DON and ZEA by increasing antioxidant levels in piglet ovaries.

CLC Number:

S828.4

CHEN Ya, WEI Quan-wei, DU Wen-chao, ZHENG Wei-jiang, DING Wei, XING Jun, SHI Fang-xiong. Mitigation of Lactulose and Hydrogen-rich Water on Ovarian Dysfunction Caused by Dietary Contaminated Corn in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2641-2651.

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Mitigation of Lactulose and Hydrogen-rich Water on Ovarian Dysfunction Caused by Dietary Contaminated Corn in Weaned Piglets

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